Manuscript Number : VC041

Development of Nano Biosensor for Cholesterol Detection

Authors(5) :-Abinayashree Nagaraj, Harshini Murugesan, Lakshmi Subash, Libin Johnson, Baalakumar

The aim of this study is to develop cholesterol biosensor using synthesized reduced Graphene Oxide (rGO) by modified Hummer’s method and reduced Graphene Oxide-Silver (rGO-Ag) nanocomposite had prepared by a simple chemical method. The rGO-Ag nanocomposite was synthesized in the presence of Silver Nitrate (AgNo3) and Sodium borohydride (NaBH4). The synthesized reduced Graphene Oxide (rGO) and reduced Graphene Oxide–silver rGO-Ag nanocomposite were characterized by Ultraviolet-Visible Spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR) and Dynamic Light Scattering (DLS). Then the Cholesterol Oxidase (ChOx) enzyme is to be immobilized on rGO-Ag poly-pyrrole network. The rGO-Ag poly-pyrrole/ChOx enzyme is coated on electrode and its electrochemical studies are to be accomplished. The bioenzyme integrated nanostructure platform is very sensitive toward cholesterol and it has a fast response time.

Authors and Affiliations

Abinayashree Nagaraj
Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
Harshini Murugesan
Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
Lakshmi Subash
Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
Libin Johnson
Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
Baalakumar
Assistant Professor Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India

Cholesterol, Enzyme, Silver Nanoparticles, rGO.

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Publication Details

Published in : Volume 5 | Issue 5 | March-April 2020
Date of Publication : 2020-03-05
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 223-226
Manuscript Number : VC041
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Abinayashree Nagaraj, Harshini Murugesan, Lakshmi Subash, Libin Johnson, Baalakumar, " Development of Nano Biosensor for Cholesterol Detection", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 5, pp.223-226, March-April-2020.
Journal URL : https://ijsrst.com/VC041
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  • Nguyen H.H., Kim M. An overview of techniques in enzyme immobilization. Appl.Sci.Converg.Technol. 2017;26:157-163.
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