Manuscript Number : IJSRST218555

Isolation of Pseudomonas species from soil sample for production of Pyoverdine and evaluation of its potential as an antimicrobial agent

Authors(5) :-Didhiti Agarwal, Dhevang Rokkala, Shreyas Tawde, Prachi Bhatia, Sejal Rathod

Pyoverdine, a fluorescent siderophore that have high- affinity for iron is produced by Pseudomonas putida and Pseudomonas aeruginosa that synthesizes it under iron-deficient growth conditions. Pseudomonas species are often encountered in diverse ecological habitats along side being censurable for nosocomial infections spread round the world. Due to these characteristics, there's a growing interest during this microbe for a spread of uses. One such is the production of Pyoverdine, it influences the uptake of iron, along with eliminating the limited iron availability condition. Pyoverdine is important for Pseudomonas putida and Pseudomonas aeruginosa to cause acute infections. Intense research and study led to the invention of Pyoverdines being a crucial source of chelating Iron. This study is predicated on Pyoverdine extracted from two different species of Pseudomonas which will act as an antimicrobial agent for various species including Escherichia coli and Staphylococcus aureus. The organism, i.e., Pseudomonas putida and Pseudomonas aeruginosa, were isolated from soil sample using medias like: Cetrimide media, King’s B media. It had been confirmed using primary biochemical tests, along side species level identification (MALDI - TOF). Isolation was followed by studying the antimicrobial activity of Pyoverdine on different organisms using antibiotics as standard for the same. Results for the tests were obtained, colonies were observed on specific media and zone of inhibition was observed on Muller Hinton plate. Comparative studies were carried out to find which organism used up Pyoverdine or Pyoverdine - Fe complex without using FeCl3 as sole standard source. Thus, these compounds can synergize with conventional antimicrobials, forming a simpler treatment with serving as a useful gizmo

Authors and Affiliations

Didhiti Agarwal
Department of Biotechnology, University of Mumbai, Kishinchand Chellaram College, Mumbai, Maharashtra, India
Dhevang Rokkala
Department of Biotechnology, University of Mumbai, Kishinchand Chellaram College, Mumbai, Maharashtra, India
Shreyas Tawde
Department of Biotechnology, University of Mumbai, Kishinchand Chellaram College, Mumbai, Maharashtra, India
Prachi Bhatia
Department of Biotechnology, University of Mumbai, Kishinchand Chellaram College, Mumbai, Maharashtra, India
Sejal Rathod
Department of Biotechnology, University of Mumbai, Kishinchand Chellaram College, Mumbai, Maharashtra, India

Pseudomonas putida, Pseudomonas aeruginosa, Pyoverdine, Antimicrobial, Siderophore

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  16. Carlos M. H. Ferreira, Ângela Vilas-Boas, Cátia A. Sousa, Helena M. V. M. Soares and Eduardo V. Soares, 2019, Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions, AMB Expr (2019) 9:78 https://doi.org/10.1186/s13568-019-0796-3.
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Publication Details

Published in : Volume 8 | Issue 5 | September-October 2021
Date of Publication : 2021-10-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 355-361
Manuscript Number : IJSRST218555
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Didhiti Agarwal, Dhevang Rokkala, Shreyas Tawde, Prachi Bhatia, Sejal Rathod, " Isolation of Pseudomonas species from soil sample for production of Pyoverdine and evaluation of its potential as an antimicrobial agent", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 8, Issue 5, pp.355-361, September-October-2021. Available at doi : https://doi.org/10.32628/IJSRST218555    
Journal URL : https://ijsrst.com/IJSRST218555
Citation Detection and Elimination     |      | |Daniel R. Kirienko, Donghoon Kang, and Natalia V. Kirienko, 2018,  Novel Pyoverdine Inhibitors Mitigate Pseudomonas aeruginosa Pathogenesis, Front Microbiol. 2018; 9: 3317.
  • Hayder Abdulrahman, Raed Obaid Saleh, 2016, Isolation and Identification of Pseudomonas aeruginosa from different sources (soil, wound, urine) and Checking its MIC with various Antibiotics, Helix Vol.4-5: 795-799 (2016).
  • Dhusi, K. Bajpai, A. Ramteke, P. W., 2018, Overcoming antibiotic resistance: Is siderophore Trojan horse conjugation an answer to evolving resistance in microbial pathogens?, Journal of controlled release: official journal of the Controlled Release Society, 269, 63–87.
  • Iain L. Lamont, Lois W. Martin, 2003, Identification and characterization of novel P yoverdine synthesis genes in Pseudomonas aeruginosa, Microbiology, volume 149, issue 4.
  • Mary E. Peek, Abhinav Bhatnagar, 1 Nael A. McCarty, 2 and Susu M. Zughaier, 2012, Pyoverdine, the Major Siderophore in Pseudomonas aeruginosa, Evades NGAL Recognition, Interdisciplinary Perspectives on Infectious Diseases,volume 2012, article ID 843509.
  • Patrick Abou Raji El Feghali and Tarek Nawas, 2018, Extraction and purification of pyocyanin: a simpler and more reliable method, MOJ Toxicol, Volume 4 Issue 6.
  • Joan L. Slonczewski, Kenyon College, 2015, Pseudomonas putida, Microbial Biorealm.
  • Sagar Aryal, 2018, Biochemical Test and Identification of Pseudomonas aeruginosa, Microbiology info. Bergey, D. H., Krieg, N. R., & Holt,
  • J. G., (1984), Bergey's manual of systematic bacteriology.
  • J.M. Meyer, 1978, The Fluorescent Pigment of Pseudomonas fluorescens Biosynthesis, Purification and Physicochemical Properties, Journal of general Microbiology(1978), issue: 107, pages: 319-328.
  • Angel R. Cueva, Oanh Pham, Aissata Diaby, Derek Fleming, Kendra P. Rumbaugh, and Gregory E. Fernandes, 2020, Pyoverdine Assay for Rapid and Early Detection of Pseudomonas aeruginosa in Burn Wounds, Applied Bio Materials, Volume 3, Issue 8, pages: 4696-5454.
  • Alok Sharma, Bhavdish N. Johri, 2003, Combat of iron-deprivation through a plant growth promoting fluorescent Pseudomonas strain GRP3A in mung bean (Vigna radiata L. Wilzeck), Microbiological. Research. (2003) Volume: 158, Pages: 77–81 .
  • Patrick Abou Raji El Feghali and Tarek Nawas, 2018, Extraction and purification of pyocyanin: a simpler and more reliable method, MOJ Toxicol, Volume 4 Issue 6.
  • Donghoon Kang, Daniel R. Kirienko, Phillip Webster, Alfred L. Fisher, and Natalia V. Kirienko, 2018, Pyoverdine, a siderophore from Pseudomonas aeruginosa, translocates into C. elegans, removes iron, and activates a distinct host response, Virulence, volume 9, No. 1, pages: 804-817.
  • Carlos M. H. Ferreira, Ângela Vilas-Boas, Cátia A. Sousa, Helena M. V. M. Soares and Eduardo V. Soares, 2019, Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions, AMB Expr (2019) 9:78 https://doi.org/10.1186/s13568-019-0796-3.
  • Dhusi, K. Bajpai, A. Ramteke, P. W., 2018, Overcoming antibiotic resistance: Is siderophore Trojan horse conjugation an answer to evolving resistance in microbial pathogens?, Journal of controlled release: official journal of the Controlled Release Society, 269, 63–87.
  • Lázaro Molina, Zulema Udaondo, Estrella Duque, Matilde Fernández, Carlos Molina-Santiago, 2014, Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital. PLoS One, 2014; 9(1): e81604.
  • Silke Peter, Philipp Oberhettinger, Leonard Schuele, Ariane Dinkelacker, Wichard Vogel, Daniela Dörfel, Daniela Bezdan, Stephan Ossowski, Matthias Marschal, Jan Liese & Matthias Willmann, 2017, Genomic characterisation of clinical and environmental Pseudomonas putida group strains and determination of their role in the transfer of antimicrobial resistance genes to Pseudomonas aeruginosa, BMC Genomics, volume 18, article no. 859, 2017.
  • Qing-Ping Hu and Jian-Guo Xu, 2011, A simple double-layered chrome azurol S agar (SDCASA) plate assay to optimize the production of siderophores by a potential biocontrol agent Bacillus, African Journal of Microbiology Research Vol. 5(25), pp. 4321-4327, 9 November, 2011.
    • " target="_blank"> BibTeX | Daniel R. Kirienko, Donghoon Kang, and Natalia V. Kirienko, 2018,  Novel Pyoverdine Inhibitors Mitigate Pseudomonas aeruginosa Pathogenesis, Front Microbiol. 2018; 9: 3317.
  • Hayder Abdulrahman, Raed Obaid Saleh, 2016, Isolation and Identification of Pseudomonas aeruginosa from different sources (soil, wound, urine) and Checking its MIC with various Antibiotics, Helix Vol.4-5: 795-799 (2016).
  • Dhusi, K. Bajpai, A. Ramteke, P. W., 2018, Overcoming antibiotic resistance: Is siderophore Trojan horse conjugation an answer to evolving resistance in microbial pathogens?, Journal of controlled release: official journal of the Controlled Release Society, 269, 63–87.
  • Iain L. Lamont, Lois W. Martin, 2003, Identification and characterization of novel P yoverdine synthesis genes in Pseudomonas aeruginosa, Microbiology, volume 149, issue 4.
  • Mary E. Peek, Abhinav Bhatnagar, 1 Nael A. McCarty, 2 and Susu M. Zughaier, 2012, Pyoverdine, the Major Siderophore in Pseudomonas aeruginosa, Evades NGAL Recognition, Interdisciplinary Perspectives on Infectious Diseases,volume 2012, article ID 843509.
  • Patrick Abou Raji El Feghali and Tarek Nawas, 2018, Extraction and purification of pyocyanin: a simpler and more reliable method, MOJ Toxicol, Volume 4 Issue 6.
  • Joan L. Slonczewski, Kenyon College, 2015, Pseudomonas putida, Microbial Biorealm.
  • Sagar Aryal, 2018, Biochemical Test and Identification of Pseudomonas aeruginosa, Microbiology info. Bergey, D. H., Krieg, N. R., & Holt,
  • J. G., (1984), Bergey's manual of systematic bacteriology.
  • J.M. Meyer, 1978, The Fluorescent Pigment of Pseudomonas fluorescens Biosynthesis, Purification and Physicochemical Properties, Journal of general Microbiology(1978), issue: 107, pages: 319-328.
  • Angel R. Cueva, Oanh Pham, Aissata Diaby, Derek Fleming, Kendra P. Rumbaugh, and Gregory E. Fernandes, 2020, Pyoverdine Assay for Rapid and Early Detection of Pseudomonas aeruginosa in Burn Wounds, Applied Bio Materials, Volume 3, Issue 8, pages: 4696-5454.
  • Alok Sharma, Bhavdish N. Johri, 2003, Combat of iron-deprivation through a plant growth promoting fluorescent Pseudomonas strain GRP3A in mung bean (Vigna radiata L. Wilzeck), Microbiological. Research. (2003) Volume: 158, Pages: 77–81 .
  • Patrick Abou Raji El Feghali and Tarek Nawas, 2018, Extraction and purification of pyocyanin: a simpler and more reliable method, MOJ Toxicol, Volume 4 Issue 6.
  • Donghoon Kang, Daniel R. Kirienko, Phillip Webster, Alfred L. Fisher, and Natalia V. Kirienko, 2018, Pyoverdine, a siderophore from Pseudomonas aeruginosa, translocates into C. elegans, removes iron, and activates a distinct host response, Virulence, volume 9, No. 1, pages: 804-817.
  • Carlos M. H. Ferreira, Ângela Vilas-Boas, Cátia A. Sousa, Helena M. V. M. Soares and Eduardo V. Soares, 2019, Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions, AMB Expr (2019) 9:78 https://doi.org/10.1186/s13568-019-0796-3.
  • Dhusi, K. Bajpai, A. Ramteke, P. W., 2018, Overcoming antibiotic resistance: Is siderophore Trojan horse conjugation an answer to evolving resistance in microbial pathogens?, Journal of controlled release: official journal of the Controlled Release Society, 269, 63–87.
  • Lázaro Molina, Zulema Udaondo, Estrella Duque, Matilde Fernández, Carlos Molina-Santiago, 2014, Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital. PLoS One, 2014; 9(1): e81604.
  • Silke Peter, Philipp Oberhettinger, Leonard Schuele, Ariane Dinkelacker, Wichard Vogel, Daniela Dörfel, Daniela Bezdan, Stephan Ossowski, Matthias Marschal, Jan Liese & Matthias Willmann, 2017, Genomic characterisation of clinical and environmental Pseudomonas putida group strains and determination of their role in the transfer of antimicrobial resistance genes to Pseudomonas aeruginosa, BMC Genomics, volume 18, article no. 859, 2017.
  • Qing-Ping Hu and Jian-Guo Xu, 2011, A simple double-layered chrome azurol S agar (SDCASA) plate assay to optimize the production of siderophores by a potential biocontrol agent Bacillus, African Journal of Microbiology Research Vol. 5(25), pp. 4321-4327, 9 November, 2011.
    • " target="_blank">RIS | Daniel R. Kirienko, Donghoon Kang, and Natalia V. Kirienko, 2018,  Novel Pyoverdine Inhibitors Mitigate Pseudomonas aeruginosa Pathogenesis, Front Microbiol. 2018; 9: 3317.
  • Hayder Abdulrahman, Raed Obaid Saleh, 2016, Isolation and Identification of Pseudomonas aeruginosa from different sources (soil, wound, urine) and Checking its MIC with various Antibiotics, Helix Vol.4-5: 795-799 (2016).
  • Dhusi, K. Bajpai, A. Ramteke, P. W., 2018, Overcoming antibiotic resistance: Is siderophore Trojan horse conjugation an answer to evolving resistance in microbial pathogens?, Journal of controlled release: official journal of the Controlled Release Society, 269, 63–87.
  • Iain L. Lamont, Lois W. Martin, 2003, Identification and characterization of novel P yoverdine synthesis genes in Pseudomonas aeruginosa, Microbiology, volume 149, issue 4.
  • Mary E. Peek, Abhinav Bhatnagar, 1 Nael A. McCarty, 2 and Susu M. Zughaier, 2012, Pyoverdine, the Major Siderophore in Pseudomonas aeruginosa, Evades NGAL Recognition, Interdisciplinary Perspectives on Infectious Diseases,volume 2012, article ID 843509.
  • Patrick Abou Raji El Feghali and Tarek Nawas, 2018, Extraction and purification of pyocyanin: a simpler and more reliable method, MOJ Toxicol, Volume 4 Issue 6.
  • Joan L. Slonczewski, Kenyon College, 2015, Pseudomonas putida, Microbial Biorealm.
  • Sagar Aryal, 2018, Biochemical Test and Identification of Pseudomonas aeruginosa, Microbiology info. Bergey, D. H., Krieg, N. R., & Holt,
  • J. G., (1984), Bergey's manual of systematic bacteriology.
  • J.M. Meyer, 1978, The Fluorescent Pigment of Pseudomonas fluorescens Biosynthesis, Purification and Physicochemical Properties, Journal of general Microbiology(1978), issue: 107, pages: 319-328.
  • Angel R. Cueva, Oanh Pham, Aissata Diaby, Derek Fleming, Kendra P. Rumbaugh, and Gregory E. Fernandes, 2020, Pyoverdine Assay for Rapid and Early Detection of Pseudomonas aeruginosa in Burn Wounds, Applied Bio Materials, Volume 3, Issue 8, pages: 4696-5454.
  • Alok Sharma, Bhavdish N. Johri, 2003, Combat of iron-deprivation through a plant growth promoting fluorescent Pseudomonas strain GRP3A in mung bean (Vigna radiata L. Wilzeck), Microbiological. Research. (2003) Volume: 158, Pages: 77–81 .
  • Patrick Abou Raji El Feghali and Tarek Nawas, 2018, Extraction and purification of pyocyanin: a simpler and more reliable method, MOJ Toxicol, Volume 4 Issue 6.
  • Donghoon Kang, Daniel R. Kirienko, Phillip Webster, Alfred L. Fisher, and Natalia V. Kirienko, 2018, Pyoverdine, a siderophore from Pseudomonas aeruginosa, translocates into C. elegans, removes iron, and activates a distinct host response, Virulence, volume 9, No. 1, pages: 804-817.
  • Carlos M. H. Ferreira, Ângela Vilas-Boas, Cátia A. Sousa, Helena M. V. M. Soares and Eduardo V. Soares, 2019, Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions, AMB Expr (2019) 9:78 https://doi.org/10.1186/s13568-019-0796-3.
  • Dhusi, K. Bajpai, A. Ramteke, P. W., 2018, Overcoming antibiotic resistance: Is siderophore Trojan horse conjugation an answer to evolving resistance in microbial pathogens?, Journal of controlled release: official journal of the Controlled Release Society, 269, 63–87.
  • Lázaro Molina, Zulema Udaondo, Estrella Duque, Matilde Fernández, Carlos Molina-Santiago, 2014, Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital. PLoS One, 2014; 9(1): e81604.
  • Silke Peter, Philipp Oberhettinger, Leonard Schuele, Ariane Dinkelacker, Wichard Vogel, Daniela Dörfel, Daniela Bezdan, Stephan Ossowski, Matthias Marschal, Jan Liese & Matthias Willmann, 2017, Genomic characterisation of clinical and environmental Pseudomonas putida group strains and determination of their role in the transfer of antimicrobial resistance genes to Pseudomonas aeruginosa, BMC Genomics, volume 18, article no. 859, 2017.
  • Qing-Ping Hu and Jian-Guo Xu, 2011, A simple double-layered chrome azurol S agar (SDCASA) plate assay to optimize the production of siderophores by a potential biocontrol agent Bacillus, African Journal of Microbiology Research Vol. 5(25), pp. 4321-4327, 9 November, 2011.
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